VEME2022 – NGS module ResponsibleMatia Prosperi and Carla Mavian TIME CONTENT SPEAKERS SUNDAY 21 AUGUST 14:00 to 16:00 Registration. 16:00 to 16:40 Welcome to VEME. Jairo Mendez, Luiz Alcantara, Tulio de Oliveira, Juan Miguel Pascale, Ana Riviere, Luis Sucre 16:40 to 17:00 Keynote lecture 1: Genomic pathogen surveillance in Panama, from research to public health, Gorgas Memorial Institute experience Alexander Martinez 17:00 to 17:20 Keynote lecture 2: PAHO Genomic Surveillance Regional Networks: genomics to support public health response for the world Juliana Leite 17:20 to 17:30 Questions & Answers A. Martinez and J. Leite 17:30 to 19:00 Keynote lecture 3: Collaboration in South Africa to help the world on the characterisation of pandemics and epidemics Tulio Oliveira 19:00 to 21:30 VEME Cocktail Monday 22 AUGUST 8:30 to 9:00 Welcome and practical instructions. L Franco and L. Alcantara 9:00 to 10:30 Introduction to next generation sequencing. R. Scheuermann 10:30 to 11:00 Coffee break. 11:00 to 12:30 Introduction to NGS software. R. Torrieri 12:30 to 14:00 Lunch. 14:00 to 15:00 Reference alignment and SNP calling. C. Mavian, T. Paisie, M. Tagliamonte 15:00 to 16:00 Software practice for reference alignment and SNP calling. C. Mavian, T. Paisie., M. Tagliamonte 16:00 to 17:00 Coffee break + poster session. 17:00 to 17:40 Keynote lecture 4: A transdisciplinary approach to pandemic preparedness A-M Vandamme 17:40 to 18:20 Keynote lecture 5: Portable DNA sequencing: impacting the response to emerging and re-emerging viral pathogens on the ground M. Giovanetti Tuesday 23 AUGUST 8:30 to 9:30 De novo assembly. H. Schmidt 9:30 to 10:30 Bacterial and Viral Bioinformatics Resource Center (BV-BRC). R. Scheuermann. 10:30 to 11:00 Coffee break. 11:00 to 12:30 Nanopore-based sequencing for routine genomics surveillance JE. San, V. Fonseca, H. Tegally 12:30 to 13:30 Lunch. 13:30 to 14:30 Nanopore-based sequencing for routine genomics surveillance JE. San, V. Fonseca, H. Tegally 14:30 to 15:30 Software practice for de novo assembly. Y. Zhang, T. Paisie, M. Tagliamonte 15:30 to 16:00 Coffee break. 16:00 to 16:50 Free computer time. Y. Zhang, T. Paisie, M. Tagliamonte, R Kato, L Paixao and S Ortiz. 16:50 to 17:10 Keynote lecture 7: Project VigenDA: Genomic Surveillance of Dengue in the Americas. Jorge L. Munoz 17:10 to 18:10 Keynote lecture 8: Putting ecology into virus evolution. Edward Holmes Wednesday 24 AUGUST 8:00 to 8:30 Keynote lecture 9: Modernizing Pathogen Surveillance with Fully Automated Genomic Sequencing and Data Ecosystem Sasan Amini 8:30 to 9:30 Transcriptomics, RNASeq, scRNAseq. R. Scheuermann 9:30 to 10:30 SARS-CoV-2 sequence analysis and mutation profiling with Genomedetective H. Tegally, JE. San, K. Deforche, V. Fonseca 10:30 to 11:00 Coffee break. 11:00 to 12:30 Keynote lecture NGS: Rapid, Fully Automated Genomic Surveillance Platform, for Actionable Insights for Public Health Laboratories Sasan Amini 12:30 to 13:30 Lunch. 13:30 to 14:30 Software practice for transcriptomics, RNASeq R. Scheuermann, Y. Zhang 14:30 to 16:00 Free computer time R. Scheuermann, Y. Zhang, R Kato, L Paixao and S Ortiz. 16:00 to 17:00 Coffee break + poster session. 17:00 to 17:40 Keynote lecture 10: Quick and Accurate identification of SARS-CoV-2 Variants and Lineages in Africa H Tegally 17:40 to 18:20 Keynote lecture 11: Modeling and forecasting virus evolution and pathogenesis in the XXI century: from genomic epidemiology to single cell sequencing M Salemi Thursday 25 August 8:00 to 8:30 Keynote lecture 6: Molecular Epidemiology based on Ampliseq Technology: SARS-CoV 2 and Beyond. Gonzalo Sepulveda 8:30 to 9:30 Metagenomics H. Schmidt. 9:30 to 10:30 Antimicrobial Resistance - AMR S. Marini 10:30 to 11:00 Coffee break. 11:00 to 12:30 Galaxy for HIV S. Pond 12:30 to 13:30 Lunch. 13:30 to 14:30 Software practice for metagenomics C. Mavian, H. Schmidt, M. Tagliamonte 14:30 to 15:30 Free computer time. C. Mavian, H. Schmidt, M. Tagliamonte, R Kato, L Paixao and S Ortiz. 15:30 to 16:00 Coffee break. 16:00 to 17:30 Free computer time. C. Mavian, M. Tagliamonte, R Kato, L Paixao and S Ortiz. 18:30 to 20:30 Course teachers dinner