VEME2024 – NGS module ResponsibleCarla Mavian and Matia Prosperi TIME CONTENT SPEAKERS SUNDAY 4 AUGUST 17:00 to 17:50 Welcome to VEME Luiz Alcantara 17:50 to 18:10 Keynote 1: Jairo Mendez Rico 18:10 to 18:30 Keynote 2: Tulio de Oliveira 18:30 to 20:00 Coffee break. Monday 5 AUGUST 07:00 to 08:00 Registration. 8:00 to 8:30 Welcome and practical instructions Luiz Alcantara, Marta Giovanetti and Vagner Fonseca 8:30 to 9:00 Introduction to next generation sequencing R. Scheuermann 9:00 to 10:00 Reference alignment: theory C. Mavian 10:00 to 10:30 Coffee break. 10:30 to 12:00 Introduction and logging in to Terra Reference alignment and SNP calling practice (Terra) C. Tomkins-Tinch, G. Mboowa 12:00 to 13:00 Lunch. 13:00 to 14:30 Reference alignment and SNP calling practice (CMD) T. Paisie, C. Mavian 14:30 to 15:30 Reference alignment: examining results T. Paisie, C. Mavian, C. Tomkins-Tinch, G. Mboowa 15:30 to 17:00 Coffee break + poster session. Tuesday 6 AUGUST 8:00 to 9:00 De novo assembly: theory H. Schmidt 9:00 to 10:00 De novo assembly practice (Terra & miniWDL) C. Tomkins-Tinch, G. Mboowa 10:00 to 10:30 Coffee break. 10:30 to 12:00 De novo assembly practice (CMD) T. Paisie, C. Mavian 12:00 to 13:00 Lunch. 13:00 to 14:30 De novo assembly: examining results T. Paisie, C. Mavian, C. Tomkins-Tinch, G. Mboowa 14:30 to 15:30 Introduction to computing C. Tomkins-Tinch 15:30 to 16:00 Coffee break. 16:00 to 17:00 Metagenomics: taxonomic classification with kraken (Terra) C. Tomkins-Tinch, G. Mboowa Wednesday 7 AUGUST 8:00 to 9:00 Metagenomics: theory C. Mavian 9:00 to 10:00 Metagenomics: taxonomy classification with kraken (CMD) C.Mavian, T. Paisie, Ortiz S 10:00 to 10:30 Coffee break. 10:30 to 12:00 Metagenomics: de novo assembly and blast Ortiz S, C. Mavian 12:00 to 13:00 Lunch. 13:00 to 14:00 Metagenomics: examining results C. Tomkins-Tinch, G. Mboowa, C. Mavian, Ortiz S 14:00 to 14:30 Illumina Innovations for Viral Genomics Leandro Ucela Alves 14:30 to 15:00 Genome detective K Deforche, V. Fonseca, J. Xavier 15:00 to 16:00 Dashboard J. Xavier, C.Mavian, T. Paisie 16:00 to 17:00 Coffee break + poster session. Hybrid Session: https://us06web.zoom.us/j/82087200228?pwd=WjRWKavQ064wYfYQ0ZeaFk9U3I1kGY.1 17:00 to 17:30 Keynote 3: Rapid spatial Expansion Beyond the Amazon Basin: Oropouche Virus joins other main arboviruses in epidemic activity across the Americas Marta Giovanetti 17:30 to 18:00 Keynote 4: 13 years of genomic surveillance in Haiti Carla Mavian 18:00 to 18:30 Keynote 5: Mass Dengue Vaccination Campaign in Dourados: A Vaccine Breakthough Case Following Tetravalent Anti-dengue Vaccination Julio Croda Thursday 8 August 8:00 to 9:00 Bulk transcriptomics: Theory R. Scheuermann 9:00 to 10:00 Bulk transcriptomics: Practice 1 R. Scheuermann, D.Pintard 10:00 to 10:30 Coffee break. 10:30 to 12:00 Bulk transcriptomics: Practice 2 R. Scheuermann, D.Pintard 12:00 to 13:00 Lunch. 13:00 to 14:30 Single cell transcriptomics: Theory R. Scheuermann 14:30 to 15:30 Single cell transcriptomics: Practice 1 D.Pintard, R. Scheuermann, J. Xavier 15:30 to 16:00 Coffee break. 16:00 to 17:30 Single cell transcriptomics: Practice 2 D.Pintard, R. Scheuermann, J. Xavier 18:30 to 20:30 Course teachers dinner