VEME2023 – NGS module ResponsibleCarla Mavian and Matia Prosperi TIME CONTENT SPEAKERS SUNDAY 20 AUGUST 16:00 to 16:40 Welcome to VEME - Stellenbosch Museum, 37 Ryneveld St. Tulio de Oliveira 17:00 to 19:00 VEME Cocktail Event Monday 21 AUGUST 08:00 to 08:30 Registration. 8:30 to 9:30 Introduction to next generation sequencing. R. Scheuermann 9:30 to 10:30 Introduction to computing D. Park 10:30 to 11:00 Coffee break. 11:00 to 12:30 Nanopore sequencing: introduction and theory S. James, M. Moir 12:30 to 14:00 Lunch. 14:00 to 15:00 Reference alignment and SNP calling: theory C. Mavian 15:00 to 16:00 Intro to Terra and reference alignment and SNP calling D. Park, C. Tomkins-Tinch 16:00 to 17:00 Coffee break + poster session. Tuesday 22 AUGUST 8:30 to 9:30 Reference alignment and SNP calling using Terra D. Park, C. Tomkins-Tinch 9:30 to 10:30 Reference alignment and SNP calling using CLI T. Paisie, L.S. Whitmore, C. Mavian 10:30 to 11:00 Coffee break. 11:00 to 12:30 De novo assembly H. Schmidt 12:30 to 13:30 Lunch. 13:30 to 14:30 De novo assembly practice using Terra D. Park, C. Tomkins-Tinch 14:30 to 15:30 De novo assembly practice using CLI T. Paisie, L.S. Whitmore, C. Mavian 15:30 to 16:00 Coffee break. 16:00 to 16:50 Free computer time. T. Paisie, L.S. Whitmore, C. Mavian Wednesday 23 AUGUST 8:30 to 9:30 Bulk Transcriptomics: theory R. Scheuermann 9:30 to 10:30 Bulk Transcriptomics: practice 1 R. Scheuermann, Z. Wallace, J. Xavier, S. James, L.S. Whitmore 10:30 to 11:00 Coffee break. 11:00 to 12:30 Bulk Transcriptomics: practice 2 R. Scheuermann, Z. Wallace, J. Xavier, S. James, L.S. Whitmore 12:30 to 13:30 Lunch. 13:30 to 14:30 Single cell transcriptomics: theory R. Scheuermann 14:30 to 15:30 Single cell transcriptomics: practice 1 R. Scheuermann, Z. Wallace, J. Xavier, S. James, L.S. Whitmore 15:30 to 16:00 Single cell transcriptomics: practice 2 R. Scheuermann, Z. Wallace, J. Xavier, S. James, L.S. Whitmore 16:00 to 17:00 Coffee break + poster session. Keynotes: Jan Mouton Learning Centre, Stellenbosch University, De Beer Rd. 17:30 to 18:45 Keynote 1: Edward Holmes 18:45 to 20:00 Keynote 2: Marion Koopmans Thursday 24 August 8:30 to 9:30 Metagenomics H. Schmidt. 9:30 to 10:30 Metagenomics: Taxonomy Classification C. Mavian, T. Paisie, D. Park, C. Tomkins-Tinch, L.S. Whitmore 10:30 to 11:00 Coffee break. 11:00 to 12:30 Genome Detective K. Deforche, V. Fonseca, S. James 12:30 to 13:30 Lunch. 13:30 to 14:30 Nextflow A. Sharma 14:30 to 15:30 Nextflow A. Sharma 15:30 to 16:00 Coffee break. 16:00 to 17:30 Free computer time A. Sharma, T. Paisie, L.S. Whitmore 18:30 to 20:30 Course teachers dinner